Cardiology>>>>>Physiology/Biochemistry
Question 5#

Low levels of gene expression can be detected by:

A. Northern blot
B. Western blot
C. Reverse transcriptase-polymerase chain reaction (RT-PCR)
D. Southern blot
E. Gene transfer

Correct Answer is C

Comment:

Reverse transcriptase-polymerase chain reaction (RT-PCR). RT-PCR is capable of finding a single copy of RNA. Northern blot analysis requires at least 5 to 10 μg of total RNA. Western blot analysis is for determining protein levels. Southern blot analysis is for genotyping and requires multiple copies of DNA. Gene transfer is not a detection method.